|
KMID : 1161420110140060670
|
|
Journal of Medicinal Food
2011 Volume.14 No. 6 p.670 ~ p.675
|
|
|
The Inhibitory Effect of Lactobacillus plantarum KY1032 Cell Extract on the Adipogenesis of 3T3-L1 Cells
|
|
Park Do-Young
Ahn Young-Tae
Huh Chul-Sung
Jeon Seon-Min
Choi Myung-Sook
|
|
|
Abstract
|
|
|
|
Some probiotics and their cell components are known to modulate lipid metabolism in vitro and/or in vivo. This study was carried out to investigate possible anti-adipogenic action of a probiotic cell extract, Lactobacillus plantarum KY1032 cell extract (KY1032-CE), in vitro using 3T3-L1 cells. Lipid regulation in the cell culture system was assessed by AdipoRed assay and Oil red O staining of intracellular lipids and real-time polymerase chain reaction and western blot analysis of adipogenesis-related factors. AdipoRed assay revealed that KY1032-CE treatment significantly decreased lipid accumulation in maturing 3T3-L1 preadipocytes in a dose-dependent manner. Oil red O staining demonstrated that KY1032-CE reduced the number of lipid-containing rounded cells. KY1032-CE down-regulated the mRNA and protein expression of four adipocyte-specific genes: peroxisome proliferator-activated receptor-γ2, CCAAT/enhancer binding protein-α, fatty acid synthase, and adipocyte-fatty acid binding protein. Accordingly, these results indicate that KY1032-CE can reduce fat mass by modulating adipogenesis in maturing preadipocytes. Further studies are needed to elucidate its mode of actions in efficacy tests of KY1032-CE in vivo.
|
|
|
KEYWORD
|
|
|
adipocyte-specific genes, adipogenesis, Lactobacillus plantarum KY1032, 3T3-L1 cells
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|